Structural insights into UbiD reversible decarboxylation. (August 2022)

• Record Type: Journal Article
• Authors: Roberts, George W.; Leys, David
• Is Part Of: Current opinion in structural biology. Volume 75(2022)
• Publication Date: 2022-08
• Subjects: AnFdc Aspergillus nigerferulic acid decarboxylase -- prFMN prenylated flavin mononucleotide -- DMAP dimethylallylphosphate -- DAMPP dimethylallylpyrophosphate; Molecular biology -- Periodicals; 570
• DOI: 10.1016/j.sbi.2022.102432
• Languages: English
• ISSNs: 0959-440X

Abstract

Abstract: The ubiquitous UbiX-UbiD system is associated with a wide range of microbial (de)carboxylation reactions. Recent X-ray crystallographic studies have contributed to elucidating the enigmatic mechanism underpinning the conversion of α, β-unsaturated acids by this system. The UbiD component utilises a unique cofactor, prenylated flavin (prFMN), generated by the bespoke action of the associated UbiX flavin prenyltransferase. Structure determination of a range of UbiX/UbiD representatives has revealed a generic mode of action for both the flavin-to-prFMN metamorphosis and the (de)carboxylation. In contrast to the conserved UbiX, the UbiD superfamily is associated with a versatile substrate range. The latter is reflected in the considerable variety of UbiD quaternary structure, dynamic behaviour and active site architecture. Directed evolution of UbiD enzymes has taken advantage of this apparent malleability to generate new variants supporting in vivo hydrocarbon production. Other applications include coupling UbiD to carboxylic acid reductase to convert alkenes into α, β-unsaturated aldehydes via enzymatic CO2 fixation. Graphical abstract: Image 1 Highlights: The UbiX-UbiD system is ubiquitous in microbes UbiD catalyses the reversible (de)carboxylation of a wide range of α, β-unsaturated acids UbiD catalysis depends on the prenylated FMN (prFMN) cofactor produced by UbiX The extensive UbiD substrate scope is reflected in considerable variation in active site structure and dynamic behaviour Directed evolution of UbiD enzymes supports new routes in alkene production and sp 2 C-H activation