Electrochemically active biofilm-enabled biosensors: Current status and opportunities for biofilm engineering. (1st October 2022)

• Record Type: Journal Article
• Authors: Hu, Yidan; Han, Xi; Shi, Liang; Cao, Bin
• Is Part Of: Electrochimica acta. Volume 428(2022)
• Publication Date: 2022-10-01
• Subjects: Electrochemically active biofilm (EAB) -- EAB-enabled biosensors -- Biofilm engineering -- Microbial fuel cell; Electrochemistry -- Periodicals; Electrochemistry, Industrial -- Periodicals; 541.37
• DOI: 10.1016/j.electacta.2022.140917
• Languages: English
• ISSNs: 0013-4686

Abstract

Highlights: A brief summary of the status of EAB-enabled biosensors is provided. Key limitations of EAB-enabled biosensors are identified. Opportunities for biofilm engineering to improve performance of EAB-enabled biosensors are discussed. Abstract: Electrochemically active biofilms (EABs) are formed by electroactive bacteria capable of exchanging electrons with electrodes. EABs have been employed as bio-elements in bioelectrochemical sensors which sense analytes of interest by converting metabolic changes to easily detectable electrical signals. Although EAB-enabled biosensors have shown promise in environmental applications, such as water quality monitoring, their most perceived practical applications are limited by low sensitivity, low specificity and short-term stability. Engineering EABs could be an effective strategy to improve the performance of EAB-enabled biosensors. In this review, we briefly introduce EAB with the focus on its extracellular electron transfer, development and matrix, as well as EAB-enabled biosensors including their general principle and potential applications. We then discuss key limitations of EAB-enabled biosensors and the opportunities that biofilm engineering may provide to address these limitations.