The establishment of clonally derived chicken embryonic fibroblast cell line (CSC) with high transfection efficiency and ability as a feeder cell. Issue 11 (4th August 2018)

• Record Type: Journal Article
• Authors: Zhao, Ruifeng; Jin, Jing; Sun, Xinyu; Jin, Kai; Wang, Man; Ahmed, Mahmoud F.; Zuo, Qisheng; Zhang, Yani; Zhao, Zhenhua; Chen, Guohong; Li, Bichun
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 11(2018)
• Page Start: 8841
• Page End: 8850
• Publication Date: 2018-08-04
• Subjects: chicken embryonic fibroblast -- feed layer cells -- mouth pipette -- single clone -- transfection; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27137
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: This study established a single cloned chicken embryonic fibroblast (CEF) cell line. It solves the main problem of the instability of a cultured primary cell and its impact on the experiment. In this study, CEF pass through this crisis and formed a continuous cell line after subculture. We isolated single postcrisis CEF by a mouth pipette under a convert microscope then established a single cloned cell line named CSC‐1‐5 which passaged continuously from 96‐well plates to 60 mm culture plates. CSC has a normal chicken diploid karyotype, no tumorigenicity, and a high G2/M phase cell ratio. We found that Fugene could mediate the transfection of CSCs efficiently; it was significantly improved compared with the primary cells. It could also promote the proliferation of chicken embryonic stem cell as a feeder layer. Abstract : In this study, we demonstrate that the postcrisis chicken embryonic fibroblast can form a stable proliferating monoclonal cell line by single‐cell cloning. The fibroblast cell line established by this method has normal cellular properties and long‐term, stable proliferative capacity. It also has a higher transfection efficiency and the value to be feeder cells.