Cell-Surface MMP-9 Protein Is a Novel Functional Marker to Identify and Separate Proangiogenic Cells from Early Endothelial Progenitor Cells Derived from CD133+ Cells. (22nd February 2016)

• Record Type: Journal Article
• Authors: Kanayasu-Toyoda, Toshie; Tanaka, Takeshi; Kikuchi, Yutaka; Uchida, Eriko; Matsuyama, Akifumi; Yamaguchi, Teruhide
• Is Part Of: Stem cells. Volume 34:Number 5(2016:May)
• Page Start: 1251
• Page End: 1262
• Publication Date: 2016-02-22
• Subjects: Angiogenesis -- Endothelial progenitor cells -- CD133 -- Cell therapy -- Matrix metalloproteinases; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2300
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: To develop cell therapies for ischemic diseases, endothelial progenitor cells (EPCs) have been expected to play a pivotal role in vascular regeneration. It is desirable to use a molecular marker that is related to the function of the cells. Here, a quantitative polymerase chain reaction array revealed that early EPCs derived from CD133 + cells exhibited significant expression of MMP-9. Some populations of early EPCs expressed MMP-9 on the cell surface and others did not. We also attempted to separate the proangiogenic fraction from early EPCs derived from CD133 + cells using a functional cell surface marker, and we then analyzed the MMP-9 + and MMP-9 − cell fractions. The MMP-9 + cells not only revealed higher invasion ability but also produced a high amount of IL-8. Moreover, the stimulative effect of MMP-9 + cells on angiogenesis in vitro and in vivo was prohibited by anti-IL-8 antibody. These data indicate that MMP-9 is one of the useful cell surface markers for the separation of angiogenic cells. Our treatment of early EPCs with hyaluronidase caused not only a downregulation of cell-surface MMP-9 but also a decrease in invasion ability, indicating that membrane-bound MMP-9, which is one of the useful markers for early EPCs, plays an important role in angiogenesis.