Protein dynamics and conformational changes explored by hydrogen/deuterium exchange mass spectrometry. (October 2019)

• Record Type: Journal Article
• Authors: Zheng, Jie; Strutzenberg, Timothy; Pascal, Bruce D; Griffin, Patrick R
• Is Part Of: Current opinion in structural biology. Volume 58(2019)
• Page Start: 305
• Page End: 313
• Publication Date: 2019-10
• Subjects: Molecular biology -- Periodicals; 570
• DOI: 10.1016/j.sbi.2019.06.007
• Languages: English
• ISSNs: 0959-440X

Abstract

Highlights: Introductory bases and equations of HDX-MS. Protein dynamics explored by HDX-MS. Representative case studies that measured by HDX-MS. Future directions of HDX-MS. Abstract : Proteins are not rigid bodies under their physiological conditions. Here we discuss a solution-phase structural proteomics technique, hydrogen deuterium exchange coupled with mass spectrometry (HDX-MS), as a means to study protein dynamics, which can complement other structural approaches. We outline the background theory and highlight the utility of HDX-MS measurements in two case studies involving a nuclear receptor and an innate immunity receptor. We also discuss emerging software advances for improving data analysis and three-dimensional visualization.