Engineering threshold-based selection systems. Issue 9 (14th July 2021)

• Record Type: Journal Article
• Authors: Pedone, Katherine H; González-Pérez, Vanessa; Leopold, Luciana E; Rasmussen, Neal R; Der, Channing J; Cox, Adrienne D; Ahmed, Shawn; Reiner, David J
• Is Part Of: G3. Volume 11:Issue 9(2021)
• Publication Date: 2021-07-14
• Subjects: EGL-1 -- BH3-only -- SMG-1 -- nonsense-mediated decay -- NMD -- 3ʹUTR -- small GTPase -- EHT 1864 -- EHT 8560 -- Pak; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1093/g3journal/jkab234
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Using model organisms to identify novel therapeutic targets is frequently constrained by pre-existing genetic toolkits. To expedite positive selection for identification of novel downstream effectors, we engineered conditional expression of activated CED-10/Rac to disrupt Caenorhabditis elegans embryonic morphogenesis, titrated to 100% lethality. The strategy of engineering thresholds for positive selection using experimental animals was validated with pharmacological and genetic suppression and is generalizable to diverse molecular processes and experimental systems.