A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody. Issue 10 (1st October 1999)

• Record Type: Journal Article
• Authors: Ko, K H; Lee, S S; Lawton, J W
• Is Part Of: Journal of clinical pathology. Volume 52:Issue 10(1999)
• Page Start: 770
• Page End: 772
• Publication Date: 1999-10-01
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.52.10.770
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM: To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum. METHODS: Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin. RESULTS: There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO. CONCLUSIONS: MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.