Nanoparticle‐Mediated Binning and Profiling of Heterogeneous Circulating Tumor Cell Subpopulations. Issue 1 (5th November 2014)

• Record Type: Journal Article
• Authors: Mohamadi, Reza M.; Besant, Justin D.; Mepham, Adam; Green, Brenda; Mahmoudian, Laili; Gibbs, Thaddeus; Ivanov, Ivaylo; Malvea, Anahita; Stojcic, Jessica; Allan, Alison L.; Lowes, Lori E.; Sargent, Edward H.; Nam, Robert K.; Kelley, Shana O.
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 1(2015)
• Page Start: 139
• Page End: 143
• Publication Date: 2014-11-05
• Subjects: cancer cell analysis -- cell sorting -- magnetic nanoparticles -- microfluidics -- tumor cells; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201409376
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters blood‐borne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial‐to‐mesenchymal transition (EMT). Abstract : Separating subpopulations of cancer cells : Circulating tumor cells (CTCs) are inherently heterogeneous, and subpopulations may exist with varying clinical significance. A new device reads out differential levels of magnetic nanoparticle binding to profile subpopulations of CTCs with varying levels of surface expression, and provides a means to profile the epithelial‐to‐mesenchymal transition in patient CTCs.