Expression of osteoprotegerin and receptor activator for the nuclear factor‐κB ligand in XACB/LV‐bFGF/MSCs transplantation for repair of rabbit femoral head defect necrosis. Issue 2 (18th October 2018)

• Record Type: Journal Article
• Authors: Peng, Wuxun; Zhang, Jian; Zhang, Fei; Zhao, Yin; Dong, Wentao
• Is Part Of: Journal of cellular biochemistry. Volume 120:Issue 2(2019)
• Page Start: 1427
• Page End: 1434
• Publication Date: 2018-10-18
• Subjects: basic fibroblast growth factor -- bone marrow stem cells -- necrosis of the femoral head -- osteoprotegerin -- receptor activator for nuclear factor‐κB ligand; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.27201
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: The aim of this study is to observe the changes in osteoprotegerin (OPG) and receptor activator for the nuclear factor‐κB ligand (RANKL) in a rabbit model, and to explore the therapeutic effect of tissue engineering bone on femoral head necrosis. A total of 60 rabbits were randomly divided into 5 groups. The necrosis model of femoral head defects was created by dexamethasone combined with a horse serum injection. The model of femoral head necrosis was reconstructed by tissue engineering bone. The protein expressions of OPG and RANKL were detected by Western blot analysis. The expression of OPG and the RANKL protein in group E was higher than that in the other 4 groups ( P < .05); there was no significant difference between groups C and D ( P > .05). The expression of OPG protein in the rabbit femoral head necrosis group was improved by xenogeneic antigens of cancellous bone/lentiviral‐basic fibroblast growth factor/mesenchymal stem cells, which were expected to be used as an effective tissue engineering material to repair the necrosis of the femoral head. Abstract : The expression of the OPG protein in the rabbit femoral head necrosis group was improved by XACB/LV‐bFGF/MSCs, and the repair effect was better than that of the other groups, indicating that XACB/LV‐bFGF/mesenchymal stem cells may potentially be used as an effective tissue engineering material to repair the necrosis of the femoral head.