Ultrafast Extraction of Proteins from Tissues Using Desorption by Impulsive Vibrational Excitation. Issue 1 (24th October 2014)

• Record Type: Journal Article
• Authors: Kwiatkowski, Marcel; Wurlitzer, Marcus; Omidi, Maryam; Ren, Ling; Kruber, Sebastian; Nimer, Refat; Robertson, Wesley D.; Horst, Andrea; Miller, R. J. Dwayne; Schlüter, Hartmut
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 1(2015)
• Page Start: 285
• Page End: 288
• Publication Date: 2014-10-24
• Subjects: mass spectrometry -- protein extraction -- proteomics -- picosecond IR laser -- tissue samples; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201407669
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL‐DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained. Abstract : Proteins can be blasted out of intact tissues and into the gas phase in less than a millisecond with a picosecond IR laser (PIRL). The proteins are desorbed by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell. PIRL‐DIVE can be used to extract significant amounts of proteins ranging in size from a few kDa up to several MDa.