Antibody to coliform antigens in urine samples from patients with symptoms of urinary tract infection. Issue 7 (July 1987)

• Record Type: Journal Article
• Authors: McKenzie, H; Young, D N
• Is Part Of: Journal of clinical pathology. Volume 40:Issue 7(1987)
• Page Start: 787
• Page End: 792
• Publication Date: 1987-07
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.40.7.787
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : An enzyme linked immunoadsorbent assay (ELISA) was used to detect IgG, IgM, and IgA antibody in urine to a crude antigenic mixture prepared from six arbitrarily chosen strains of common coliform urinary pathogens. In a group of patients with symptoms of urinary tract infection 24 of 80 (30%) urine samples were positive on culture by conventional methods, 58 of 80 (72.5%) were positive for polymorphs on microscopy, and 72 of 80 (90%) were positive for antibody by ELISA. Secretory component was detected in 28 of 80 (35%) samples, and this is consistent with local antibody production. The results show that antibody can be detected in a wide range of urine samples using a simple antigen mixture, and this may form the basis of a useful test to diagnose urinary tract infection.