Use of peroxidase-conjugated antiglobulin as an alternative to immunofluorescence for the detection of antinuclear factor in serum. Issue 6 (September 1971)

• Record Type: Journal Article
• Authors: Dorling, J.; Johnson, G. D.; Webb, Janet A.; Smith, Mavis E.
• Is Part Of: Journal of clinical pathology. Volume 24:Issue 6(1971)
• Page Start: 501
• Page End: 505
• Publication Date: 1971-09
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.24.6.501
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : A method of detecting serum antinuclear factor by the use of antiglobulin conjugated with horseradish peroxidase has been compared with a standard procedure using a fluorescent label. The horseradish peroxidase conjugate was evaluated by block titration against positive serum and by its performance as a test reagent for screening and titrating sera. It is concluded that the horse-radish peroxidase method, in which an ordinary light microscope is employed, provides a satisfactory alternative to immunofluorescence for routine tests for antinuclear factor.