Development of quantitative analytical method for volatile thiol compound with LC-ESI-MS as nonvolatile derivative by integrating a thiol-specific derivatization. Issue 9 (19th July 2021)

• Record Type: Journal Article
• Authors: Kawano, Yusuke; Suzuki, Kengo; Ohtsu, Iwao
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 9(2021)
• Page Start: 1932
• Page End: 1936
• Publication Date: 2021-07-19
• Subjects: 2-furanmethanethiol -- LC-ESI-MS -- volatile thiol compounds -- monobromobimane -- coffee aroma; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab129
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Generally, volatile thiols are hard to be measured with electrospray-ionization-type LC-MS due to the volatility. Therefore, we here evaluated the pretreatment of their S -bimanyl derivatization by monobromobimane to enable the detection as nonvolatile derivative. Consequently, we successfully developed the convenient and efficient method through the quantitative analysis of 2-furanmethanethiol (volatile thiol odorant of coffee aroma) in coffee bean.