Functional kaurene-synthase-like diterpene synthases lacking a gamma domain are widely present in Oryza and related species. Issue 9 (9th July 2021)

• Record Type: Journal Article
• Authors: Itoh, Akira; Nakazato, Shinta; Wakabayashi, Hiroki; Hamano, Ayame; Shenton, Matthew R; Miyamoto, Koji; Mitsuhashi, Wataru; Okada, Kazunori; Toyomasu, Tomonobu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 9(2021)
• Page Start: 1945
• Page End: 1952
• Publication Date: 2021-07-09
• Subjects: biosynthesis -- evolution -- diterpene synthase -- rice -- wild species; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab127
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Various diterpene synthases have been functionally identified in cultivated rice ( Oryza sativa ). These are the homologs of ent- copalyl diphosphate ( ent- CDP) synthase and ent- kaurene synthase (KS) that are responsible for the biosynthesis of gibberellins, diterpenoid phytohormones. We isolated a cDNA encoding full-length OsKSL12, a previously uncharacterized KS like (KSL) enzyme that consists of a β-domain and an α-domain with an active center, but lacks an N-terminal γ-domain. Functional analysis using a bacterial expression system showed that recombinant OsKSL12 converted ent- CDP into ent- manool or ent -13- epi -manool. Comparative genomics revealed that functional OsKSL12 homologs exist in diverse wild species in the Oryzeae— Oryza nivara ( Oryza rufipogon ), Oryza coarctata, Oryza granulata, Leersia perrieri, and Leersia tisseranti . KSL12 homologs in O. granulata, L. perrieri, and L. tisseranti preferentially reacted with geranylgeranyl diphosphate rather than ent -CDP, resulting in geranyllinalool rather than ent -manool or ent -13- epi -manool as the main product, meaning that KSL12 functionally diversified during evolution in the Oryzeae. Graphical Abstract: