Large-scale preparation of sialyl-Tn antigen-containing peptides from mucin-like glycoproteins in boar seminal gel. Issue 9 (23rd June 2021)

• Record Type: Journal Article
• Authors: Takeuchi, Ryota; Maeda, Megumi; Nakano, Miran; Funahashi, Hiroaki; Kimura, Yoshinobu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 9(2021)
• Page Start: 2022
• Page End: 2025
• Publication Date: 2021-06-23
• Subjects: boar seminal gel -- mucin-like glycoproteins -- O-glycans -- sialyl-Tn antigens; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab114
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Sialyl-Tn antigen, a tumor antigen, is a valuable ligand for the purification of proteins that specifically bind to it. Here, we developed a new method for the preparation of large amounts of sialyl-Tn antigen-containing peptides from an unused resource, boar seminal gel. The glycopeptides were prepared from the actinase E digests by a combination of gel filtration and hydrophilic partitioning.