Eight genes are necessary and sufficient for biogenesis of quinohemoprotein amine dehydrogenase. Issue 9 (30th June 2021)

• Record Type: Journal Article
• Authors: Nakai, Tadashi; Tanizawa, Katsuyuki; Okajima, Toshihide
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 85:Issue 9(2021)
• Page Start: 2026
• Page End: 2029
• Publication Date: 2021-06-30
• Subjects: quinohemoprotein amine dehydrogenase -- post-translational modification -- biogenesis of cofactor -- heterologous expression -- amine assimilation; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1093/bbb/zbab117
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: Quinohemoprotein amine dehydrogenase (QHNDH) containing a peptidyl quinone cofactor, cysteine tryptophylquinone, is produced in the periplasm of Gram-negative bacteria through an intricate process of post-translational modification that requires at least 8 genes including those encoding 3 nonidentical subunits and 3 modifying enzymes. Our heterologous expression study has revealed that the 8 genes are necessary and sufficient for the QHNDH biogenesis.