Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model. Issue 1 (July 1997)

• Record Type: Journal Article
• Authors: Kabir, A M; Aiba, Y; Takagi, A; Kamiya, S; Miwa, T; Koga, Y
• Is Part Of: Gut. Volume 41:Issue 1(1997)
• Page Start: 49
• Page End: 55
• Publication Date: 1997-07
• Subjects: Gastroenterology -- Periodicals; 616.33
• DOI: 10.1136/gut.41.1.49
• Languages: English
• ISSNs: 0017-5749

Abstract

Abstract : BACKGROUND: Helicobacter pylori is a bacterium which causes gastric inflammatory diseases. Oral inoculation of H pylori usually results in only a temporary colonisation without a successful infection in the stomach of conventional mice in which lactobacilli are the predominant indigenous bacteria. AIM: To determine whether lactobacilli exert an inhibitory effect on colonisation by H pylori in the stomach. METHODS: The effects of H pylori on attachment to murine and human gastric epithelial cells and the H pylori mediated release of interleukin-8 (IL-8) by these cells were examined in vitro. Lactobacillus salivarius infected gnotobiotic BALB/c mice and control germ free mice were inoculated orally with H pylori to examine whether L salivarius can inhibit colonisation by H pylori. RESULTS: L salivarius inhibited both the attachment and IL-8 release in vitro. H pylori could not colonise the stomach of L salivarius infected gnotobiotic BALB/c mice, but colonised in large numbers and subsequently caused active gastritis in germ free mice. In addition, L salivarius given after H pylori implantation could eliminate colonisation by H pylori. CONCLUSION: These findings suggest the possibility of lactobacilli being used as probiotic agents against H pylori.