Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost‐Effective Diagnosis of Infectious Disease. Issue 46 (30th September 2015)

• Record Type: Journal Article
• Authors: Park, Ki Soo; Charles, Richelle C.; Ryan, Edward T.; Weissleder, Ralph; Lee, Hakho
• Is Part Of: Chemistry. Volume 21:Issue 46(2015)
• Page Start: 16359
• Page End: 16363
• Publication Date: 2015-09-30
• Subjects: aptamer -- biosensors -- DNA polymerase -- infectious disease -- nucleic acid testing; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201502934
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract: A new nucleic acid detection method was developed for a rapid and cost‐effective diagnosis of infectious disease. This approach relies on the three unique elements: 1) detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2) universal reporters conjugated with a single fluorophore; and 3) fluorescence polarization (FP) detection. As a proof‐of‐concept, the assay was used to detect and sub‐type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours. Abstract : Nucleic acid testing : Detection probes that regulate DNA polymerase activity were rationally designed for a rapid and cost‐effective diagnosis of infectious disease. This assay system was successfully utilized to detect and sub‐type Salmonella bacteria with sensitivities down to a single bacterium in relatively short periods of time (see scheme).