Abnormal expression of liver autophagy and apoptosis-related mRNA in fatty liver haemorrhagic syndrome and improvement function of resveratrol in laying hens. Issue 2 (3rd March 2020)

• Record Type: Journal Article
• Authors: Wang, Xiaoyu; Xing*, Chenghong; Yang, Fan; Zhou, Sihui; Li, Guyue; Zhang, Caiying; Cao, Huabin; Hu, Guoliang
• Is Part Of: Avian pathology. Volume 49:Issue 2(2020)
• Page Start: 171
• Page End: 178
• Publication Date: 2020-03-03
• Subjects: FLHS -- resveratrol -- liver -- apoptosis -- autophagy -- gene expression; Poultry -- Diseases -- Periodicals; Birds -- Diseases -- Periodicals; 571.918
• DOI: 10.1080/03079457.2019.1698712
• Languages: English
• ISSNs: 0307-9457

Abstract

ABSTRACT: Fatty liver haemorrhagic syndrome (FLHS) is characterized by hepatic rupture and haemorrhage leading to sudden death in laying hens. Resveratrol (Res) is a natural polyphenol with antioxidant and anti-inflammatory effects that can ameliorate chronic liver disease. The aim of this study was to investigate the improved effect of Res on the altered expression of autophagy and apoptosis-related genes in laying hens with FLHS. A total of 144 healthy 150-day-old laying hens were randomly divided into four groups: control group (standard diet), HELP group (high-energy-low-protein (HELP) diet), HELP + Res group (HELP diet with 400 mg/kg Res) and Res group (standard diet with 400 mg/kg Res). Histopathological lesions of the liver and the mRNA levels of Beclin-1, Atg5, Atg7, p62, Bcl-2, Bax and Caspase-3 on days 40, 80, and 120 were measured. The results showed that lipid accumulation and hepatocyte damage in the HELP group were more serious than those in the HELP + Res group. The mRNA levels of Beclin-1, Atg5, Atg7, and Bcl-2 in the HELP and HELP + Res groups were strikingly declined ( P < 0.01) compared to the control group, and their mRNA levels were markedly higher in HELP group than those in the HELP + Res group ( P < 0.05). Additionally, the mRNA levels of p62, Bax and Caspase-3 were significantly increased in the HELP and HELP + Res groups ( P < 0.01 or P < 0.05), but their mRNA levels in the HELP group were higher than those in the HELP + Res group ( P < 0.05). Collectively, FLHS could induce severe lipid accumulation, abnormal mRNA levels of liver autophagy and apoptosis-related genes. Res as a dietary supplement could attenuate these abnormal changes.