Biochar enhanced high-solid mesophilic anaerobic digestion of food waste: Cell viability and methanogenic pathways. (June 2021)

• Record Type: Journal Article
• Authors: Cui, Yuxuan; Mao, Feijian; Zhang, Jingxin; He, Yiliang; Tong, Yen Wah; Peng, Yinghong
• Is Part Of: Chemosphere. Volume 272(2021)
• Publication Date: 2021-06
• Subjects: Biochar -- High-solid anaerobic digestion -- Food waste -- Cell viability -- Methanogenic pathways; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.129863
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The underlying mechanisms of biochar enhance high-solid anaerobic digestion (HSAD) of food waste were investigated with a focus on the cell viability, microbial community, and methanogenic pathways. This study assessed the effects of different dosages of biochar in HSAD. Optimal biochar dosage was found to be 25 g/L, which produced accumulative methane yields of up to 251 mL CH4 /g VS significantly promote volatile fatty acid degradations, especially in butyric acid concentrations. Effects of biochar with a dosage of 25 g/L on the cell viability showed that viable cells based on cell membrane integrity increased from 2.9% to 6.4%. Meanwhile, intact and highly active cells with high DNA content were probably involved in direct interspecies electron transfer (DIET) via membrane-bound electron transport proteins. Further analysis demonstrated that Syntrophomonas and methanogens Methanosarcina & Methanocelleus were selectively enriched by biochar, which resulted in the methanogenic pathways shifting from acetoclastic/hydrogenotrophic methanogenic pathways to more metabolically diverse methanogenic pathways. Accordingly, biochar-mediated DIET was possibly established between Syntrophomonas and Methanosarcina species due to those viable cells. In conclusion, biochar is a feasible additive in enhancing HSAD methanogenic performance. Graphical abstract: Image 1 Highlights: Novel insights into cell viability of bacteria in HSAD were provided. Biochar enhanced HSAD performance with max methane yield of up to 251 mL CH4 /g VS. Viable cells based on cell membrane integrity may be involved in DIET. More metabolically diverse methanogenic pathways were found with biochar assistance. Biochar-mediated DIET possibly established between Syntrophomonas and Methanosarcina.