A Tryptophanyl‐tRNA Synthetase/tRNA Pair for Unnatural Amino Acid Mutagenesis in E. coli. Issue 19 (2nd April 2013)

• Record Type: Journal Article
• Authors: Chatterjee, Abhishek; Xiao, Han; Yang, Peng‐Yu; Soundararajan, Gautam; Schultz, Peter G.
• Is Part Of: Angewandte Chemie international edition. Volume 52:Issue 19(2013)
• Page Start: 5106
• Page End: 5109
• Publication Date: 2013-04-02
• Subjects: amber suppression -- genetic code expansion -- unnatural amino acids -- protein engineering; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201301094
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : Selected : A tryptophanyl‐tRNA synthetase(TrpRS)/tRNA pair was evolved to genetically encode tryptophan analogues and other unnatural amino acids with large side chains in E. coli. A selection scheme was employed to identify TrpRS variants able to selectively charge tRNACUA Trp with Trp analogues (see picture). Substitution of Trp66 in enhanced cyan fluorescent protein (ECFP) with these Trp analogues afforded ECFP variants with novel spectral properties.