Cover Picture: A Rapid, Amplification‐Free, and Sensitive Diagnostic Assay for Single‐Step Multiplexed Fluorescence Detection of MicroRNA (Angew. Chem. Int. Ed. 34/2015). Issue 34 (29th July 2015)

• Record Type: Journal Article
• Authors: Jin, Zongwen; Geißler, Daniel; Qiu, Xue; Wegner, K. David; Hildebrandt, Niko
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 34(2015)
• Page Start: 9725
• Page End: 9725
• Publication Date: 2015-07-29
• Subjects: clinical diagnostics -- FRET -- microRNA -- multiplexing -- time‐gated fluorescence detection; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201506281
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract : The simple, sensitive, and selective detection of short‐sequence microRNAs is very challenging. In their Communication on page 10024 ff. N. Hildebrandt and co‐workers present a single‐step "mix‐and‐measure" FRET assay for the multiplexed detection of different microRNAs. The careful design of the biophotonic probe and time‐gated FRET from a luminescent Tb complex to three difference fluorescent dyes are crucial to this microRNA test for multiplexed clinical diagnostics.