A Genome Assembly of the Barley 'Transformation Reference' Cultivar Golden Promise. Issue 6 (1st June 2020)

• Record Type: Journal Article
• Authors: Schreiber, Miriam; Mascher, Martin; Wright, Jonathan; Padmarasu, Sudharasan; Himmelbach, Axel; Heavens, Darren; Milne, Linda; Clavijo, Bernardo J; Stein, Nils; Waugh, Robbie
• Is Part Of: G3. Volume 10:Issue 6(2020)
• Page Start: 1823
• Page End: 1827
• Publication Date: 2020-06-01
• Subjects: Barley -- reference assembly -- Golden Promise; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.119.401010
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Barley ( Hordeum vulgare ) is one of the most important crops worldwide and is also considered a research model for the large-genome small grain temperate cereals. Despite genomic resources improving all the time, they are limited for the cv . Golden Promise, the most efficient genotype for genetic transformation. We have developed a barley cv . Golden Promise reference assembly integrating Illumina paired-end reads, long mate-pair reads, Dovetail Chicago in vitro proximity ligation libraries and chromosome conformation capture sequencing (Hi-C) libraries into a contiguous reference assembly. The assembled genome of 7 chromosomes and 4.13Gb in size, has a super-scaffold N50 after Chicago libraries of 4.14Mb and contains only 2.2% gaps. Using BUSCO (benchmarking universal single copy orthologous genes) as evaluation the genome assembly contains 95.2% of complete and single copy genes from the plant database. A high-quality Golden Promise reference assembly will be useful and utilized by the whole barley research community but will prove particularly useful for CRISPR-Cas9 experiments.