Exploring rotavirus proteome to identify potential B- and T-cell epitope using computational immunoinformatics. Issue 12 (December 2020)

• Record Type: Journal Article
• Authors: Devi, Yengkhom Damayanti; Devi, Arpita; Gogoi, Hemanga; Dehingia, Bondita; Doley, Robin; Buragohain, Alak Kumar; Singh, Ch. Shyamsunder; Borah, Partha Pratim; Rao, C.Durga; Ray, Pratima; Varghese, George M.; Kumar, Sachin; Namsa, Nima D.
• Is Part Of: Heliyon. Volume 6:Issue 12(2020)
• Publication Date: 2020-12
• Subjects: Rotavirus -- Immune epitope -- Structural proteins -- Non-structural proteins; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2020.e05760
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: Rotavirus is the most common cause of acute gastroenteritis in infants and children worldwide. The functional correlation of B- and T-cells to long-lasting immunity against rotavirus infection in the literature is limited. In this work, a series of computational immuno-informatics approaches were applied and identified 28 linear B-cells, 26 conformational B-cell, 44 TC cell and 40 TH cell binding epitopes for structural and non-structural proteins of rotavirus. Further selection of putative B and T cell epitopes in the multi-epitope vaccine construct was carried out based on immunogenicity, conservancy, allergenicity and the helical content of predicted epitopes. An in-silico vaccine constructs was developed using an N-terminal adjuvant (RGD motif) followed by TC and TH cell epitopes and B-cell epitope with an appropriate linker. Multi-threading models of multi-epitope vaccine construct with B- and T-cell epitopes were generated and molecular dynamics simulation was performed to determine the stability of designed vaccine. Codon optimized multi-epitope vaccine antigens was expressed and affinity purified using the E. coli expression system. Further the T cell epitope presentation assay using the recombinant multi-epitope constructs and the T cell epitope predicted and identified in this study have not been investigated. Multi-epitope vaccine construct encompassing predicted B- and T-cell epitopes may help to generate long-term immune responses against rotavirus. The computational findings reported in this study may provide information in developing epitope-based vaccine and diagnostic assay for rotavirus-led diarrhea in children's. Abstract : Rotavirus; immune epitope; structural proteins; non-structural proteins.