Structures and inhibitory activity against breast cancer cells of new bufadienolides from the eggs of toad Bufo bufo gargarizans. Issue 96 (30th September 2016)

• Record Type: Journal Article
• Authors: Zhang, Peng-Wei; Tian, Hai-Yan; Nie, Qiu-Lin; Wang, Lei; Zhou, Shi-Wen; Ye, Wen-Cai; Zhang, Dong-Mei; Jiang, Ren-Wang
• Is Part Of: RSC advances. Volume 6:Issue 96(2016)
• Page Start: 93832
• Page End: 93841
• Publication Date: 2016-09-30
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra18676a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : New bufadienolides with inhibitory activity against breast cancer cells were isolated from the eggs of toad Bufo bufo gargarizans . Abstract : Two new 19-norbufadienolides (1 and 2 ), one new 14, 15-epoxy bufadienolide (3 ), and eight rare bufadienolide–fatty acid conjugates (4–11 ), together with four known ones (12–15 ) were isolated from the eggs of toad Bufo bufo gargarizans . Their chemical structures were elucidated by extensive spectroscopic methods in combination with X-ray diffraction analyses. Furthermore, we tested the inhibitory effect of these compounds against breast cancer cell lines MCF-7 and MDA-MB-231. Most of them showed strong cytotoxicity with IC50 values less than 0.1 μM. The further mechanistic study showed that they could induce apoptosis and cycle arrest in the G2 /M phase in MCF-7 cells.