The identification of salicylates as normal constituents of serum: a link between diet and health?. Issue 7 (July 1998)

• Record Type: Journal Article
• Authors: Paterson, J R; Blacklock, C; Campbell, G; Wiles, D; Lawrence, J R
• Is Part Of: Journal of clinical pathology. Volume 51:Issue 7(1998)
• Page Start: 502
• Page End: 505
• Publication Date: 1998-07
• Subjects: Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.51.7.502
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : AIM: To examine sera for the presence of salicylic acid and 2, 3- and 2, 5-dihydroxybenzoic acids (2, 3- and 2, 5-DHBA), in individuals not taking salicylate drugs. METHODS: Extracts of acidified serum samples were analysed by high performance liquid chromatography with electro-chemical detection. The chromatographic conditions were altered, and the retention times of the unknown compounds compared against authentic salicylic acid, 2, 3-DHBA, and 2, 5-DHBA. Serum samples (some spiked with salicylic acid) were incubated with salicylate hydroxylase and analyses undertaken. An extract of acidified serum was derivatised using N-methyl-N-trimethylsilyltrifluoroacetamide and the salicylic acid derivative identified by gas chromatography-mass spectrometry. RESULTS: Salicylic acid, 2, 3-DHBA, and 2, 5-DHBA were identified as being normal constituents of serum. CONCLUSIONS: Salicylic acid, 2, 3-DHBA, and 2, 5-DHBA possess anti-inflammatory properties. The finding that these compounds are present as normal constituents of serum, possibly arising from diet, raises important questions as to their role in the promotion of health.