Dual staining of corneal endothelium with trypan blue and alizarin red S: importance of pH for the dye-lake reaction. Issue 12 (December 1981)

• Record Type: Journal Article
• Authors: Taylor, M. J.; Hunt, C. J.
• Is Part Of: British journal of ophthalmology. Volume 65:Issue 12(1981)
• Page Start: 815
• Page End: 819
• Publication Date: 1981-12
• Subjects: Ophthalmology -- Periodicals; 617.7
• DOI: 10.1136/bjo.65.12.815
• Languages: English
• ISSNs: 0007-1161

Abstract

Abstract : Evaluation of corneal endothelial integrity by combined staining with the vital stain trypan blue and the intercellular stain alizarin red S provides a simple, quick technique for visualisation of both damaged and normal cells, thereby permitting the quantification of endothelial cell damage. Adjustment of the pH of the alizarin red S reagent to 4.2 is important for optimum dye-laking at the intercellular borders, and brief fixation with glutaraldehyde maintains the staining effect of both dyes.