Genome and transcriptome of a pathogenic yeast, Candida nivariensis. Issue 7 (23rd April 2021)

• Record Type: Journal Article
• Authors: Fan, Yunfan; Gale, Andrew N; Bailey, Anna; Barnes, Kali; Colotti, Kiersten; Mass, Michal; Morina, Luke B; Robertson, Bailey; Schwab, Remy; Tselepidakis, Niki; Timp, Winston
• Editors: Andrews, B J
• Is Part Of: G3. Volume 11:Issue 7(2021)
• Publication Date: 2021-04-23
• Subjects: Candida nivariensis -- genome assembly -- pathogenic yeast -- nanopore sequencing -- transcriptome; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1093/g3journal/jkab137
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis . We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata .