Application Effect and Accuracy Analysis of Electrochemiluminescence Immunoassay and Enzyme-Linked Immunosorbent Assay in the Serological Test of Hepatitis B Virus. (4th August 2022)

• Record Type: Journal Article
• Authors: Lian, Yifei; Fu, Hongran; Xu, Xiaoyi; Ju, Chuanyu
• Other Names: Wang Tian Jiao Academic Editor.
• Is Part Of: Evidence-based complementary and alternative medicine. Volume 2022(2022)
• Publication Date: 2022-08-04
• Subjects: Alternative medicine -- Periodicals; 615.505
• DOI: 10.1155/2022/9371497
• Languages: English
• ISSNs: 1741-427X

Abstract

Abstract : Objective . To explore the validity and accuracy of electrochemiluminescence immunoassay (ECLIA) and enzyme-linked immunosorbent assay (ELISA) in the serological detection of the hepatitis B virus. Methods . From 6 February 2019 to 1 March 2020, 96 patients diagnosed with hepatitis B virus infection in our hospital were recruited and assigned at a ratio of 1 : 1 to experimental groups A (GA) and B (GB), with 48 cases in each group, and the five major serological indicators of hepatitis B were tested and analyzed using ECLIA and ELISA. In addition, 50 suspected patients were selected for two tests, respectively, to compare the accuracy of the two test methods. Results . ECLIA was associated with significantly higher expression levels and higher detection rates of HBeAg, HBeAb, HBsAg, and HBsAb versus ELISA (P < 0.05 ), and the difference in the expression and detection rates of HBcAb levels between the two groups did not come up to the statistical standard (P > 0.05 ). ECLIA yielded significantly higher sensitivity and specificity than ELISA (P < 0.05 ), while the two methods showed comparable detection accuracy (P > 0.05 ). Conclusion . Despite the inconsistent results of the latest studies on the serological detection of hepatitis B by the two techniques, ECLIA is consistently superior to ELISA and provides better diagnostic benefits and merits promotion.