New granule cells in the olfactory bulb are associated with high respiratory input in an enriched odor environment. (September 2022)

• Record Type: Journal Article
• Authors: Kamimura, Sawa; Masaoka, Yuri; Yoshikawa, Akira; Kamijo, Shotaro; Ohtaki, Hirokazu; Koiwa, Nobuyoshi; Honma, Motoyasu; Sakikawa, Kei; Kobayashi, Hitome; Izumizaki, Masahiko
• Is Part Of: Neuroscience research. Volume 182(2022)
• Page Start: 52
• Page End: 59
• Publication Date: 2022-09
• Subjects: BrdU 5-bromo-2′-deoxyuridine -- DG dentate gyrus -- GCL granule cell layer -- MCL mitral cell layer; Olfaction -- Respiration -- Olfactory bulb -- Mitral cell -- Granule cell -- Dentate gyrus -- New cell -- Odor-enriched environment; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2022.05.007
• Languages: English
• ISSNs: 0168-0102

Abstract

Abstract: New neurons are constantly generated in the olfactory bulb and the dentate gyrus of the hippocampus. The number of new cells depends on sensory experiences; an enriched odor environment increases neurogenesis and neural survival. The aim of this study was to investigate whether enriched olfactory stimuli affect neurogenesis of mitral and granule cells of the olfactory bulb and dentate gyrus, and whether respiratory activity accompanied by olfactory stimuli is associated with new cells in these regions. To this end, respiratory activity during enriched odor stimuli was continuously measured in mice and new cells were stained with 5-bromo-2′-deoxyuridine, which selectively labels proliferating cells. An enriched olfactory environment significantly increased neurogenesis of mitral and granule cells in the olfactory bulb, but not in the dentate gyrus. Additionally, an increase of new granule cells under the enriched odor condition was correlated to sniffing frequency power, which had a significantly different pattern from the no-odor condition. A high respiratory frequency with frequent odor stimuli may be associated with activation of granule cells to form inhibitory neurons and this active state might increase granule cell neurogenesis. Highlights: Enriched odor environment increases granule cell neurogenesis in the olfactory bulb. Enriched odor environment increases mitral cell neurogenesis in the olfactory bulb. The increase in new granule cells was correlated with sniffing frequency. Animals with a high sniff frequency in response to odor had increased granule cell neurogenesis.