A single lymphocyte culture for fragile X induction and prometaphase chromosome analysis. Issue 12 (December 1991)

• Record Type: Journal Article
• Authors: Griffiths, M J; Strachan, M C
• Is Part Of: Journal of medical genetics. Volume 28:Issue 12(1991)
• Page Start: 837
• Page End: 839
• Publication Date: 1991-12
• Subjects: Medical genetics -- Periodicals; 616.042
• DOI: 10.1136/jmg.28.12.837
• Languages: English
• ISSNs: 1468-6244

Abstract

Abstract : A single lymphocyte culture system is described which produces both reliable fragile X expression and elongated chromosomes for prometaphase analysis. This system, which is based on that described by Wheater and Roberts in 1987, involves the deoxycytidine release of a thymidine block. Eight fragile X positive subjects had an average expression level of 26%, with a range of 12% to 45%, using the thymidine/deoxycytidine protocol. This was comparable to the levels obtained in parallel cultures treated with thymidine alone or fluorodeoxyuridine.