Postnatal development of the microstructure of cortical GABAergic synapses and perineuronal nets requires sensory input. (September 2022)

• Record Type: Journal Article
• Authors: Lipachev, Nikita; Melnikova, Anastasia; Fedosimova, Svetlana; Arnst, Nikita; Kochneva, Anastasia; Shaikhutdinov, Nurislam; Dvoeglazova, Anastasia; Titova, Angelina; Mavlikeev, Mikhail; Aganov, Albert; Osin, Yuri; Kiyasov, Andrei; Paveliev, Mikhail
• Is Part Of: Neuroscience research. Volume 182(2022)
• Page Start: 32
• Page End: 40
• Publication Date: 2022-09
• Subjects: Perineuronal nets -- GABAergic synapses -- Postnatal development -- Quantitative analysis; Neurosciences -- Research -- Periodicals; Neurosciences -- Research -- Japan -- Periodicals; Neurology -- Periodicals; Neurosciences -- Periodicals; Neurosciences -- Recherche -- Périodiques; Neurosciences -- Recherche -- Japon -- Périodiques; Neurosciences -- Research; Japan; Periodicals; 612.8
• DOI: 10.1016/j.neures.2022.06.005
• Languages: English
• ISSNs: 0168-0102

Abstract

Abstract: The brain synaptic circuitry is formed as a result of pre-defined genetic programs and sensory experience during postnatal development. Perineuronal nets ensheath synaptic boutons and control several crucial features of the synapse physiology. Formation of the perineuronal net microstructure during the brain development remains largely unstudied. Here we provide a detailed quantitative description of the 3-dimensional geometry of the synapse and the surrounding perineuronal net in the mouse somatosensory cortex layer IV. We compare the morphology of the synapse+perineuronal net complex in the adult brain formed under normal conditions or in the whisker shaving model of somatosensory deprivation. We demonstrate that the sensory deprivation causes flattening of the 3D PNN mesh geometry and reduction of the VGAT-positive cluster volume in presynaptic boutons. These results reveal a mechanism of the sensory input-dependent synapse morphogenesis during the brain development. Highlights: Here we propose new methods for quantitative analysis of the 3D microstructure of perineuronal nets. Somatosensory deprivation via whisker shaving causes flattening of the perineuronal nets. Somatosensory deprivation also affects microstructure of the GABAergic synapses surrounded by perineuronal nets as shown by reduction of the volume of VGAT-positive clusters.