Characterization of Bacillus velezensis E2 with abilities to degrade ochratoxin A and biocontrol against Aspergillus westerdijkiae fc-1. (September 2022)

• Record Type: Journal Article
• Authors: Zhang, Yiming; Li, Zhenchao; Lu, Yenan; Zhang, Jiaqi; Sun, Yemei; Zhou, Jiayu; Tu, Tingting; Gong, Weifeng; Sun, Weihong; Wang, Yun
• Is Part Of: Toxicon. Volume 216(2022)
• Page Start: 125
• Page End: 131
• Publication Date: 2022-09
• Subjects: Aspergillus westerdijkiae -- Bacillus velezensis -- Biodegradation -- Ochratoxin A; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2022.07.006
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: Ochratoxin A (OTA), primarily produced by the fungi belonging to the species of Aspergillus and Penicillium, is one of the most common mycotoxins found in cereals and fruits. In addition to resulting in huge economic losses, OTA contamination also poses considerable threat to human and livestock health. Microbial degradation of mycotoxins has been considered with great potential in mycotoxins decontamination. In a previous study, Bacillus velezensis E2 was isolated by our laboratory and showed appreciable inhibitory effect on Aspergillus flavus growth and aflatoxin production in rice grains. In this study, B. velezensis E2 was investigated for its ability to remove OTA and biocontrol against the ochratoxigenic Aspergillus westerdijkiae fc-1. The results revealed that B. velezensis E2 has considerable inhibitory effect on A. westerdijkiae fc-1 both on PDA medium and pear fruits, with inhibitory rate of 51.7% and 73.9%, respectively. In addition, its ability to remove OTA was evaluated in liquid medium and the results showed that more than 96.1% of OTA with an initial concentration of 2.5 μg/mL could be removed by B. velezensis E2 in 48 h. Further experiments revealed that enzymatic transformation and alkaline hydrolysis might be the main mechanisms related to OTA degradation by B. velezensis E2, with ring open ochratoxin α (OP-OTα) as a possible degradation product. Our study indicated that the B. velezensis E2 strain could be a potential bacterial candidate in biodegradation of OTA and biocontrol against A. westerdijkiae fc-1. Graphical abstract: Image 1 Highlights: Bacillus velezensis E2 exhibits antifungal activity against Aspergillus westerdijkiae fc-1. Bacillus velezensis E2 can remove ochratoxin A mainly through degradation. Ring open ochratoxin α is a tentative degradation product of ochratoxin A.