Multiple exposure to methylmercury aggravates DNA damage in the BTBR T + Itpr3 tf/J autistic mouse model: The role of DNA repair efficiency. (July 2022)

• Record Type: Journal Article
• Authors: Attia, Sabry M.; Ahmad, Sheikh F.; Nadeem, Ahmed; Attia, Mohamed S.M.; Ansari, Mushtaq A.; Al-Hamamah, Mohammed A.; Hussein, Marwa H.; Alameen, Alaa A.; Alasmari, Abdullah F.; Bakheet, Saleh A.
• Is Part Of: Toxicology. Volume 477(2022)
• Publication Date: 2022-07
• Subjects: Autism -- Methylmercury -- Genomic damage -- Oxidative stress -- DNA repair; Toxicology -- Periodicals; Chemicals -- Physiological effect -- Periodicals; 615.9005
• DOI: 10.1016/j.tox.2022.153277
• Languages: English
• ISSNs: 0300-483X

Abstract

Abstract: Environmental and genetic factors have been recognized to play major roles in the pathogenesis of autism. Here we examined the BTBR T + Itpr3 tf /J (BTBR) mice's susceptibility, an autistic model, to the genotoxic effects and DNA repair dysregulation of methylmercury. Micronuclei formation and oxidative DNA damage were analyzed using the micronucleus/fluorescence in situ hybridization test and modified comet assay, respectively. The results showed higher centromeric-positive micronuclei and oxidative DNA damage in BTBR mice exposed to methylmercury than the unexposed mice, which indicates that mutagenesis aggravated in BTBR mice after methylmercury exposure. Lipid peroxides in BTBR mice were significantly elevated, with a decrease in reduced/oxidized glutathione ratio after methylmercury exposure, indicating an augmenting oxidant-antioxidant imbalance. The expression of several genes involved in DNA repair was markedly altered in BTBR mice after methylmercury exposure as evaluated via PCR array and RT-PCR analyses. Declining of the antioxidant defense and dysregulation in DNA repair process after methylmercury exposure may explain the aggravated genotoxic susceptibility of BTBR mice. Thus, autistic individuals exposed to methylmercury must be under regular medical follow-up through standard timetabled medical laboratory inquiry to allow for early recognition of any mutagenic changes. Additionally, strategies that elevate cellular antioxidants/DNA repair efficiency may counteract methylmercury-induced genotoxicity. Highlights: Methylmercury worsens DNA repair gene disturbances in BTBR mice. Methylmercury worsens oxidant/antioxidant imbalance in BTBR mice BTBR mice showed a higher sensitivity to induced genomic damage than C57BL/6 J mice. Health prophylactic measures to avert delayed effects of methylmercury are needed.s