Puerarin protects against H2O2-induced apoptosis of HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/Akt axis. (August 2022)

• Record Type: Journal Article
• Authors: He, Lidan; Wu, Xiuyan; Zhang, Xia; Li, Xuemei; Lin, Xin; Huang, Yameng; Wu, Jianbo
• Is Part Of: Placenta. Volume 126(2022)
• Page Start: 202
• Page End: 208
• Publication Date: 2022-08
• Subjects: Preeclampsia -- Puerarin -- miR-20a-5p -- MMP2 -- VEGFA; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2022.06.015
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Preeclampsia seriously affects the health of pregnant women and fetuses. It has been reported that puerarin has a positive therapeutic effect on the treatment of preeclampsia. In this study, oxidative stress-induced trophoblast cell injury was established to explore the potential interaction between puerarin and preeclampsia. Methods: A CCK-8 assay was performed to investigate the effect of puerarin on the viability of HTR-8/SVneo cells. To mimic oxidative stress-induced trophoblast cell injury, human villous trophoblasts (HTR-8/SVneo) were treated with H2 O2 . Then, the relationships among MMP2, VEGFA and miR-20a-5p in HTR-8/SVneo cells were confirmed using a dual-luciferase reporter assay. Finally, Western blot assays were performed to measure the expression levels of MMP2, VEGFA, p-Akt, Akt, Bcl-2 and cleaved caspase 3. Results: In this study, puerarin eliminated H2 O2 -induced cytotoxicity of HTR-8/SVneo cells. In addition, puerarin was able to reverse H2 O2 -induced apoptosis and metastasis inhibition in cells. Meanwhile, puerarin significantly abrogated H2 O2 -induced mitochondrial membrane potential (MMP) decline in HTR-8/SVneo cells. And, MMP2 and VEGFA were identified as direct targets of miR-20a-5p. Furthermore, puerarin reversed H2 O2 -induced growth inhibition in HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/Akt axis. Discussion: All these data indicated that puerarin could abolish H2 O2 -induced growth inhibition in HTR-8/SVneo cells by regulating the miR-20a-5p/VEGFA/AKT axis. Highlights: Puerarin could eliminate H2 O2 -induced cytotoxicity of HTR-8/SVneo cells. Puerarin was able to reverse H2O2-induced apoptosis and metastasis inhibition in cells. MMP2 and VEGFA were identified as the direct targets of miR-20a-5p. Puerarin significantly abrogated H2O2-induced mitochondrial membrane potential (MMP) decline in HTR-8/SVneo cells. Puerarin reversed H2O2-induced growth inhibition in HTR-8/SVneo cells via regulating miR-20a-5p/VEGFA/Akt axis.