Associations between antenatal maternal asthma status and placental DNA methylation. (August 2022)

• Record Type: Journal Article
• Authors: Lee, Alison G.; Tignor, Nicole; Cowell, Whitney; Colicino, Elena; Bozack, Anne; Baccarelli, Andrea; Wang, Pei; Wright, Rosalind J.
• Is Part Of: Placenta. Volume 126(2022)
• Page Start: 184
• Page End: 195
• Publication Date: 2022-08
• Subjects: Maternal antenatal asthma -- Placenta -- DNA methylation -- Differentially variable positions -- Sex-specific effects; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2022.06.008
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Maternal asthma in pregnancy is associated with adverse perinatal and child health outcomes; however, mechanisms are poorly understood. Methods: The PRogramming of Intergenerational Stress Mechanisms (PRISM) prospective pregnancy cohort characterized asthma history during pregnancy via questionnaires and quantified placental DNAm using the Illumina Infinium HumanMethylation450 BeadChip. We performed epigenome-wide association analyses (n = 223) to estimate associations between maternal active or inactive asthma, as compared to never asthma, and placental differentially methylated positions (DMPs) and differentially variable positions (DVPs). Models adjusted for maternal pre-pregnancy body mass index, smoking status, parity, age and education level and child sex. P-values were FDR-adjusted. Results: One hundred and fifty-nine (71.3%) pregnant women reported no history of asthma (never asthma), 15 (6.7%) reported inactive, and 49 (22%) reported active antenatal asthma. Women predominantly self-identified as Black/Hispanic Black [88 (39.5%)] and Hispanic/non-Black [42 (18.8%)]. We identified 10 probes at FDR<0.05 and 4 probes at FDR<0.10 characterized by higher variability in maternal active asthma compared to never asthma mapping to GPX3, LHPP, PECAM1, ATAD3C, and ARHGEF4 and 2 probes characterized by lower variation mapping to CHMP4A and C5orf24 . Amongst women with inactive asthma, we identified 52 probes, 41 at FDR<0.05 and an additional 11 at FDR <0.10, with higher variability compared to never asthma; BMP4, LHPP, PHYHIPL, and ZSCAN23 were associated with multiple DVPs. No associations were observed with DMPs. Discussion: We observed alterations in placental DNAm in women with antenatal asthma, as compared to women without a history of asthma. Further research is needed to understand the impact on fetal development. Highlights: Prior evidence suggests that placental DNA methylation impacts placental function and fetal development. Maternal asthma was found to alter placental DNA methylation, specifically differentially variable positions. Involved genes are related to placental function and embryonic development. Exploratory analyses suggested a sex-dependent effect only in comparing mothers with inactive vs never asthma.