Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy. (28th February 2015)

• Record Type: Journal Article
• Authors: Schmidt-Lucke, Caroline; Escher, Felicitas; Van Linthout, Sophie; Kühl, Uwe; Miteva, Kapka; Ringe, Jochen; Zobel, Thomas; Schultheiss, Heinz-Peter; Tschöpe, Carsten
• Other Names: D'Acquisto Fulvio Academic Editor.
• Is Part Of: Mediators of inflammation. Volume 2015(2015)
• Publication Date: 2015-02-28
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1155/2015/308185
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Introduction . Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods . In 29 patients with n = 23 or without n = 6 CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1 α mRNA expression were detected via immunohistochemistry and real-time PCR. Results . MSC defined as CD45 − CD34 − CD11b − CD73 + CD90 + cells accounted for 0.010 [0.0025–0.048]%/peripheral mononuclear cell (PMNC) and as CD45 − CD34 − CD11b − CD73 + CD105 + cells for 0.019 [0.0026–0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% P < 0.01 transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (P < 0.05, multivariate analysis). A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45 − CD34 − CD90 + CD105 + ) in EMB (r = - 0.73, P < 0.005 ). SDF-1 α was the strongest predictor for increased MSC in EMB (P < 0.005, multivariate analysis). Conclusions . Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation.