Canonical ammonia oxidizers, rather than comammox Nitrospira, dominated autotrophic nitrification during the mineralization of organic substances in two paddy soils. (May 2021)

• Record Type: Journal Article
• Authors: Liu, Haiyang; Hu, Hangwei; Huang, Xing; Ge, Tida; Li, Yongfu; Zhu, Zhenke; Liu, Xingmei; Tan, Wenfeng; Jia, Zhongjun; Di, Hongjie; Xu, Jianming; Li, Yong
• Is Part Of: Soil biology and biochemistry. Volume 156(2021)
• Publication Date: 2021-05
• Subjects: Autotrophic nitrification -- Ammonia-oxidizing bacteria -- Ammonia-oxidizing archaea -- Comammox -- Organic substances; Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2021.108192
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Chemoautotrophic canonical ammonia-oxidizers and complete ammonia oxidizers (comammox) are responsible for ammonia oxidation, which is the rate-limiting step of nitrification in terrestrial ecosystems. However, the relative importance of autotrophic nitrification and the related active nitrifiers during mineralization of organic substances with different C/N ratios have not been fully elucidated to date. In this study, 15 N tracing and 13 CO2 -DNA-based stable isotope probing (DNA-SIP) techniques were employed to investigate the responses of soil nitrification processes and the active nitrifying phylotypes in acidic and neutral paddy soils with amendment of glutamine (Gln) or rice straw with low C/N (LS) or high C/N (HS). Acetylene (C2 H2 ) was utilized to differentiate autotrophic and heterotrophic nitrification. The results indicated that C2 H2 completely blocked the production of 15 NO3 − in Gln and LS treatments, implying the predominance of autotrophic nitrification with the N derived from the mineralization of Gln and LS. Neither 15 NH4 + nor 15 NO3 − was detected in the HS-amended soils, suggesting that NH4 + derived from organic N mineralization was immobilized immediately by microorganisms. In the DNA-SIP microcosms, the abundance of ammonia-oxidizing bacteria (AOB) in the unamended control (CK), Gln and LS treatments of acidic paddy soil increased by 22.1-, 71.9- and 27.6-fold, respectively. A significantly larger proportion of Nitrosospira -like AOB, rather than ammonia-oxidizing archaea (AOA) and comammox Nitrospira, was labelled by 13 CO2, indicating that AOB made a stronger contribution to autotrophic nitrification in acidic paddy soil. The active nitrification of neutral paddy soil was predominated by AOA affiliated with the Nitrososphaera viennensis lineage in the CK and LS microcosms but by Nitrosospira -like AOB in the Gln microcosm. These results suggest the significance of autotrophic nitrification catalysed by canonical ammonia oxidizers, rather than comammox, during the mineralization of organic substances with low C/N in paddy soils. Graphical abstract: Image 1 Highlights: Gln and low C/N rice straw stimulated the autotrophic nitrification in paddy soils. AOB rather than AOA and comammox dominated nitrification in acidic paddy soil. AOA dominated nitrification with mineralization of SOM or straw in neutral soil. Nitrosospira cluster 3 was the active AOB in acidic and neutral paddy soils.