Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis. (24th December 2017)

• Record Type: Journal Article
• Authors: Chapman, Victoria; Markides, Hareklea; Sagar, Devi Rani; Xu, Luting; Burston, James J.; Mapp, Paul; Kay, Alasdair; Morris, Robert H.; Kehoe, Oksana; El Haj, Alicia J.
• Other Names: Carriel Víctor Academic Editor.
• Is Part Of: Stem cells international. Volume 2017(2017)
• Publication Date: 2017-12-24
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2017/2905104
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : Background . Mesenchymal stem cells (MSCs) have a therapeutic potential for the treatment of osteoarthritic (OA) joint pathology and pain. The aims of this study were to determine the influence of a passage number on the effects of MSCs on pain behaviour and cartilage and bone features in a rodent model of OA. Methods . Rats underwent either medial meniscal transection (MNX) or sham surgery under anaesthesia. Rats received intra-articular injection of either 1.5 × 10 6 late passage MSCs labelled with 10 μ g/ml SiMAG, 1.5 × 10 6 late passage mesenchymal stem cells, the steroid Kenalog (200 μ g/20 μ L), 1.5 × 10 6 early passage MSCs, or serum-free media (SFM). Sham-operated rats received intra-articular injection of SFM. Pain behaviour was quantified until day 42 postmodel induction. Magnetic resonance imaging (MRI) was used to localise the labelled cells within the knee joint. Results . Late passage MSCs and Kenalog attenuated established pain behaviour in MNX rats, but did not alter MNX-induced joint pathology at the end of the study period. Early passage MSCs exacerbated MNX-induced pain behaviour for up to one week postinjection and did not alter joint pathology. Conclusion . Our data demonstrate for the first time the role of a passage number in influencing the therapeutic effects of MSCs in a model of OA pain.