CircRNA‐associated ceRNA network construction reveals the circRNAs involved in the progression and prognosis of breast cancer. Issue 4 (15th October 2019)

• Record Type: Journal Article
• Authors: Ma, Xiaoran; Liu, Cun; Gao, Chundi; Li, Jie; Zhuang, Jing; Liu, Lijuan; Li, Huayao; Wang, Xue; Zhang, Xiaoming; Dong, Shixia; Zhou, Chao; Sun, Changgang
• Is Part Of: Journal of cellular physiology. Volume 235:Issue 4(2020:Apr.)
• Page Start: 3973
• Page End: 3983
• Publication Date: 2019-10-15
• Subjects: breast cancer -- ceRNA network -- circRNAs -- Gene Set Enrichment Analysis -- Weighted gene co‐expression network analysis; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.29291
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Recently, increasing evidences show that circular RNAs (circRNAs) are important regulators of various diseases, especially cancer. However, the regulatory role and the potential mechanism of action of circRNAs in breast cancer remain largely unknown. In this study, weighted gene co‐expression network analysis was conducted with the differentially expressed miRNAs and mRNAs in breast cancer from The Cancer Genome Atlas database to identify the key modules associated with the carcinogenesis of breast cancer. In the significant turquoise and brown modules, 22 miRNAs and 1877 mRNAs were identified, respectively. Then, We compared and predicted the target genes and performed survival analysis to identify the miRNAs and mRNAs related to the prognosis of breast cancer. A circRNA‐related competitive endogenous RNA network was identified by database co‐screening, and deleted in liver cancer 1 (DLC1) was identified as a key gene. Finally, to assess how genes in key modules and key genes contribute to the development of breast cancer, relevant pathway information was obtained through DAVID and Gene Set Enrichment Analysis. These data demonstrated that three circRNAs (hsa‐circ‐0083373, hsa‐circ‐0083374, and hsa‐circ‐0083375) that regulate DLC1 expression via hsa‐mir‐511 and are involved in the pathogenesis and development of breast cancer. Abstract : Breast cancer is the most frequently diagnosed cancer in women. Despite considerable efforts directed to improve the survival and quality of life of patients, the treatment and prognosis of breast cancer are still unsatisfactory owing to high recurrence rate and poor prognosis. In this study, a competitive endogenous RNA (ceRNA) network was constructed based on the data from patients with breast cancer obtained from The Cancer Genome Atlas (TCGA) and related RNA databases. We found that three circRNAs (hsa‐circ‐0083373, hsa‐circ‐0083374, and hsa‐circ‐0083375) regulated deleted in liver cancer 1 (DLC1) expression via hsa‐mir‐511 and were involved in the pathogenesis and development of breast cancer. This study provides a better understanding of the molecular mechanism underlying breast cancer development and may help identify novel therapeutic strategies and biomarkers.