Multimarker Screening of Oxidative Stress in Aging. (16th July 2014)

• Record Type: Journal Article
• Authors: Syslová, Kamila; Böhmová, Adéla; Mikoška, Miloš; Kuzma, Marek; Pelclová, Daniela; Kačer, Petr
• Other Names: Cao Weiguo Academic Editor.
• Is Part Of: Oxidative medicine and cellular longevity. Volume 2014(2014)
• Publication Date: 2014-07-16
• Subjects: Oxidative stress -- Periodicals; Cells -- Aging -- Periodicals; Cells -- Aging; Oxidative stress; Oxidative Stress -- Periodicals; Cell Aging -- Periodicals; Periodicals; 611.0181
• DOI: 10.1155/2014/562860
• Languages: English
• ISSNs: 1942-0900

Abstract

Abstract : Aging is a complex process of organism decline in physiological functions. There is no clear theory explaining this phenomenon, but the most accepted one is the oxidative stress theory of aging. Biomarkers of oxidative stress, substances, which are formed during oxidative damage of phospholipids, proteins, and nucleic acids, are present in body fluids of diseased people as well as the healthy ones (in a physiological concentration). 8- iso prostaglandin F2 α is the most prominent biomarker of phospholipid oxidative damage, o -tyrosine, 3-chlorotyrosine, and 3-nitrotyrosine are biomarkers of protein oxidative damage, and 8-hydroxy-2′-deoxyguanosine and 8-hydroxyguanosine are biomarkers of oxidative damage of nucleic acids. It is thought that the concentration of biomarkers increases as the age of people increases. However, the concentration of biomarkers in body fluids is very low and, therefore, it is necessary to use a sensitive analytical method. A combination of HPLC and MS was chosen to determine biomarker concentration in three groups of healthy people of a different age (twenty, forty, and sixty years) in order to find a difference among the groups.