A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli. (15th October 2014)

• Record Type: Journal Article
• Authors: Elloumi-Mseddi, Jihene; Jellali, Karim; Villalobo, Antonio; Aifa, Sami
• Other Names: Manna Adhar C. Academic Editor.
• Is Part Of: TheScientificWorldjournal. Volume 2014(2014)
• Publication Date: 2014-10-15
• Subjects: Science -- Periodicals; Technology -- Periodicals; Medicine -- Periodicals; 505
• DOI: 10.1155/2014/830923
• Languages: English
• ISSNs: 2356-6140

Abstract

Abstract : Our success in producing an active epidermal growth factor receptor (EGFR) tyrosine kinase in Escherichia coli encouraged us to express the full-length receptor in the same host. Despite its large size, we were successful at producing the full-length EGFR protein fused to glutathione S-transferase (GST) that was detected by Western blot analysis. Moreover, we obtained a majoritarian truncated GST-EGFR form detectable by gel electrophoresis and Western blot. This truncated protein was purified and confirmed by MALDI-TOF/TOF analysis to belong to the N-terminal extracellular region of the EGFR fused to GST. Northern blot analysis showed two transcripts suggesting the occurrence of a transcriptional arrest.