Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells. Issue 1 (23rd August 2017)

• Record Type: Journal Article
• Authors: Zhang, Chen; Wang, Man; He, Nana; Ahmed, Mahmoud F.; Wang, Yilin; Zhao, Ruifeng; Yu, Xinjian; Jin, Jing; Song, Jiuzhou; Zuo, Qisheng; Zhang, Yani; Li, Bichun
• Is Part Of: Journal of cellular biochemistry. Volume 119:Issue 1(2018)
• Page Start: 1111
• Page End: 1121
• Publication Date: 2017-08-23
• Subjects: chicken -- differentiation -- HSD3B2 -- male germ cells -- steroid hormone pathway; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.26279
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: Steroid hormones regulate differentiation of various types of cell during embryogenesis. Testosterone is one of the androgens that bind to receptors to regulate gene expression and promote spermatogenesis. Our results showed that testosterone, as a product of steroid hormones synthesis pathway, could facilitate the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs). The analysis of the steroid hormones synthesis pathway demonstrated that 3beta‐hydroxysteroid dehydrogenase2 ( Hsd3b2 ) plays a major role in the synthesis of testosterone. In the absence of Hsd3b2, the expression of downstream genes such as Cyp1a1, Ugt1a1, and Hsd17b7 was not maintained. This reduction is probably due to the down‐regulation of the steroid hormones synthesis pathway. Furthermore, qRT‐PCR, immunofluorescence, and flow cytometry analysis confirmed that the steroid hormones synthesis pathway could facilitate the differentiation of ESCs. Altogether, these results lead to a model in which Hsd3b2 regulates ESCs differentiation via modulating the activity of steroid hormones synthesis pathway. Abstract : Testosterone was confirmed to be able to induce the differentiation of SSCs. HSD3B2 could regulates the differentiation of ESCs by modulating the steroid hormone synthesis pathway.