MicroRNA‐19a attenuates hypoxia‐induced cardiomyocyte apoptosis by downregulating NHE‐1 expression and decreasing calcium overload. Issue 2 (21st October 2019)

• Record Type: Journal Article
• Authors: Ma, Jiaqi; Chen, Zhangwei; Ma, Yuanji; Xia, Yan; Hu, Kai; Zhou, You; Chen, Ao; Qian, Juying; Ge, Junbo
• Is Part Of: Journal of cellular biochemistry. Volume 121:Issue 2(2020)
• Page Start: 1747
• Page End: 1758
• Publication Date: 2019-10-21
• Subjects: apoptosis -- calcium overload -- cardiomyocytes -- hypoxia -- microRNA‐19a -- NHE‐1; Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.29411
• Languages: English
• ISSNs: 0730-2312

Abstract

Abstract: miR‐19a has been shown to be involved in coronary microvascular obstruction injury; however, the underlying molecular mechanisms remain unknown. In our study, we tried to explore the role of miR‐19a in cardiomyocyte apoptosis and calcium overload in vivo and in vitro induced by hypoxia. We established the acute myocardial infarction (AMI) rat model by ligating the left anterior descending artery. The expression of miR‐19a in the infarct zone of AMI rats and myocardial tissue in the same position in sham rats was analyzed using RT‐qPCR while Na (+) /H (+) exchanger 1 (NHE‐1) was detected by Western blotting. We also observed the effects of overexpressing miR‐19a or administering an NHE‐1 inhibitor (cariporide) on hypoxia‐induced (HI) calcium overload and apoptosis in primary cardiomyocytes. In addition, dual‐luciferase reporter assays were conducted to investigate the potential target of miR‐19a on NHE‐1. Decreased miR‐19a expression, as well as increased apoptosis and NHE‐1 expression, were observed in the AMI model. Furthermore, after hypoxia stimulation, miR‐19a was gradually reduced as time increased in primary cardiomyocytes. Overexpressing miR‐19a using mimics ameliorated the increase in NHE‐1 in hypoxic cardiomyocytes and thereby reduced the HI cell calcium overload and cell apoptosis rate from 12.32% to 9.5% ( P < .01). In addition, the dual‐luciferase reporter gene assay results verified that NHE‐1 was the direct target of miR‐19a. Our findings suggest that miR‐19a activation can attenuate HI cardiomyocyte apoptosis by downregulating NHE‐1 expression and decreasing calcium overload.