PLZF suppresses differentiation of mouse spermatogonial progenitor cells via binding of differentiation associated genes. Issue 3 (20th September 2019)

• Record Type: Journal Article
• Authors: Song, Weixiang; Shi, Xinglong; Xia, Qin; Yuan, Min; Liu, Jiaxi; Hao, Kunying; Qian, Yinjuan; Zhao, Xiaodong; Zou, Kang
• Is Part Of: Journal of cellular physiology. Volume 235:Issue 3(2020:Mar.)
• Page Start: 3033
• Page End: 3042
• Publication Date: 2019-09-20
• Subjects: binding motif -- ChIP‐qPCR -- promoter -- STRA8 -- transcription suppressor; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.29208
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Promyelocytic leukaemia zinc finger (PLZF) is a key factor in inhibiting differentiation of spermatogonial progenitor cells (SPCs), but the underlying mechanisms are still largely unknown. In this study, the regulation of PLZF on Kit, Stra8, Sohlh2, and Dmrt1 (SPCs differentiation related genes) was investigated. We found some PLZF potential binding sites existed in the promoters of Kit, Stra8, Sohlh2, and Dmrt1 . Additionally, the expressions of KIT, STRA8, SOHLH2, and DMRT1 were upregulated when PLZF was knockdown in SPCs. Furthermore, chromatin immunoprecipitation quantitative polymerase chain reaction revealed PLZF directly bound to the promoters of Kit, Stra8, Sohlh2, and Dmrt1 . Besides, dual luciferase assay verified PLZF repressed those gene expressions. Collectively, our finding indicate that PLZF binds to the promoter regions of Kit, Stra8, Sohlh2, and Dmrt1 to regulate SPCs differentiation, which facilitate us to further understand the regulatory mechanism of PLZF in SPCs fates. Abstract : Whether can transcription suppressor promyelocytic leukaemia zinc finger (PLZF) directly bind to the differentiation marker gene Kit in spermatogonial progenitor cells (SPCs) is controversial topic. This study reveals PLZF regulates differentiation associated genes Kit, Sohlh2, Stra8, and Dmrt1 via direct binding to their promoter regions, which help us to further understand the regulatory mechanism of SPCs differentiation.