Skewed macrophage polarization in aging skeletal muscle. Issue 6 (2nd September 2019)

• Record Type: Journal Article
• Authors: Cui, Chang‐Yi; Driscoll, Riley K.; Piao, Yulan; Chia, Chee W.; Gorospe, Myriam; Ferrucci, Luigi
• Is Part Of: Aging cell. Volume 18:Issue 6(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-09-02
• Subjects: aging -- IMAT -- macrophages -- polarization -- skeletal muscle; Cells -- Aging -- Periodicals; 571.8783605
• DOI: 10.1111/acel.13032
• Languages: English
• ISSNs: 1474-9718

Abstract

Abstract: Skeletal muscle aging is a major cause of disability and frailty in the elderly. The progressive impairment of skeletal muscle function with aging was recently linked to a disequilibrium between damage and repair. Macrophages participate in muscle tissue repair, first as pro‐inflammatory M1 subtype and then as anti‐inflammatory M2 subtype. However, information on the presence of macrophages in skeletal muscle is still sporadic and the effect of aging on macrophage phenotype remains unknown. In this study, we sought to characterize the polarization status of macrophages in skeletal muscle of persons across a wide range of ages. We found that most macrophages in human skeletal muscle are M2, and that this number increased with advancing age. On the contrary, M1 macrophages declined with aging, making the total number of macrophages invariant with older age. Notably, M2 macrophages colocalized with increasing intermuscular adipose tissue (IMAT) in aging skeletal muscle. Similarly, aged BALB/c mice showed increased IMAT and M2 macrophages in skeletal muscle, accompanied by slightly increased collagen protein production. Collectively, we report that polarization of macrophages to the major M2 subtype is associated with IMAT and propose that increased M2 in aged skeletal muscle may impact upon muscle metabolism associated with aging. Abstract : With advancing age, M2 macrophages were found to accumulate in skeletal muscle, particularly in the vicinity of intermuscular adipose tissue (IMAT). In addition, IMAT and regenerating muscle fibers increased with age. Given that IMAT secretes M2‐skewing cytokines IL13 and IL4, we propose a connection between M2 polarization and IMAT and suggest that the rise in M2 macrophages may be an adaptive response to repair aged skeletal muscle.