Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructured particles. (30th January 2019)

• Record Type: Journal Article
• Authors: Gaspar, Diana P; Vital, Joana; Leiva, María C; Gonçalves, Lídia MD; Taboada, Pablo; Remuñán-López, Carmen; Vítor, Jorge; Almeida, António J
• Is Part Of: Nanomedicine. Volume 14:Number 4(2019)
• Page Start: 407
• Page End: 429
• Publication Date: 2019-01-30
• Subjects: chitosan -- gene delivery -- inhalation -- microencapsulation -- plasmid -- solid lipid nanoparticles -- spray-drying; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0270
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Cationically modified solid lipid nanoparticles (SLN) were investigated as plasmid DNA ( pDNA ) carriers and transfection agents for the pulmonary route.Materials & methods: pDNA -loaded SLN were produced using glyceryl dibehenate or tristearate as matrix lipids and chitosan as surface charge modifier, and encapsulated by spray-drying in mannitol and trehalose microspheres.Results: Nanoparticles of 200 nm, and zeta potential around +15 mV were produced. Electrophorectic analysis confirmed plasmid stability and integrity. The pDNA -loaded SLN were able to transfect the Calu-3 and A549 pulmonary cell lines, while showing low cytotoxicity. Microencapsulation of SLN yielded dry powders suitable for inhalation that protected pDNA from degradation.Conclusion: Microencapsulated SLN are a promising safe and effective carrier system for pulmonary gene delivery following pulmonary administration.