Effects of silver nanoparticles functionalized with Cornus mas L. extract on architecture and apoptosis in rat testicle. (18th January 2019)

• Record Type: Journal Article
• Authors: Opris, Razvan; Toma, Vlad; Olteanu, Diana; Baldea, Ioana; Baciu, Alina Mihaela; Lucaci, Florica Imre; Berghian-Sevastre, Alexandra; Tatomir, Corina; Moldovan, Bianca; Clichici, Simona; David, Luminita; Florea, Adrian; Filip, Gabriela Adriana
• Is Part Of: Nanomedicine. Volume 14:Number 3(2019)
• Page Start: 275
• Page End: 299
• Publication Date: 2019-01-18
• Subjects: apoptosis -- electron microscopy -- rat testicle -- silver nanoparticles -- transcription factors; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2018-0193
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: To assess ultrastructural changes, alterations in matrix metalloproteinase activity and apoptosis induced by silver nanoparticles (AgNPs) in the rat testicle.Materials & methods: For 45 days, two groups of animals received different doses of AgNPs (0.8 and 1.5 mg/kg b.w.), and a control group was given the buffer used as vehicle for AgNPs. At 7 and 15 days post-treatment, transmission electron microscopy, TUNEL assay, evaluation of NFkB, pNFkB, p53, Bcl-2 and Nrf2 expressions were performed on the removed testes.Results: Transmission electron microscopy revealed severe ultrastructural changes of interstitial tissue and seminiferous epithelium sustained by positive signal for apoptosis. The promatrix metalloproteinase-2 activity and NFkB, Bcl-2 expressions were increased, mainly at 7 days.Conclusion: AgNPs induced severe cell lesions identified even a long time after the exposure.