Analysis of some immunogenic properties of the recombinant Sporothrix schenckii Gp70 expressed in Escherichia coli. (11th March 2019)

• Record Type: Journal Article
• Authors: Martínez-Álvarez, José A; García-Carnero, Laura C; Kubitschek-Barreira, Paula H; Lozoya-Pérez, Nancy E; Belmonte-Vázquez, José L; de Almeida, José RF; J Gómez-Infante, Antonio de; Curty, Nathalia; Villagómez-Castro, Julio C; Peña-Cabrera, Eduardo; Martínez-Duncker, Iván; Almeida, Sandro R; Lopes-Bezerra, Leila M; Mora-Montes, Héctor M
• Is Part Of: Future microbiology. Volume 14:Number 5(2019)
• Page Start: 397
• Page End: 410
• Publication Date: 2019-03-11
• Subjects: antibodies -- cell wall -- glycoprotein -- Gp70 -- immunity -- immunodetection -- Sporothrix schenckii; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0295
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: Sporothrix schenckii is the causative agent of sporotrichosis. A 70-kDa glycoprotein, Gp70, is a candidate for the development of prophylactic alternatives to control the disease, and its gene ( GP70 ) is predicted to encode for a protein of 43 kDa, contrasting with the molecular weight of the native protein.Materials & methods: The GP70 was expressed in bacteria, the recombinant protein purified, used in immunoassays and injected to Galleria mellonella .Results & conclusion: The recombinant protein was detected by anti-Gp70 antibodies, confirming that the Gp70 backbone is a 43-kDa peptide. This protein showed enzyme activity of cyclase and was recognized by sera of patients with sporotrichosis. Although it was not useful for serodiagnosis of sporotrichosis, it conferred protection to animals against experimental sporotrichosis.