First fungemia case due to environmental yeast Wickerhamomyces myanmarensis: detection by multiplex qPCR and antifungal susceptibility. (12th March 2019)

• Record Type: Journal Article
• Authors: Arastehfar, Amir; Bakhtiari, Mina; Daneshnia, Farnaz; Fang, Wenjie; Sadati, Sara Khanjari; Al-Hatmi, Abdullah MS; Groenewald, Marizeth; Sharifi-Mehr, Hamid; Liao, Wanqing; Pan, Weihua; Zomorodian, Kamiar; Hagen, Ferry; Boekhout, Teun
• Is Part Of: Future microbiology. Volume 14:Number 4(2019)
• Page Start: 267
• Page End: 274
• Publication Date: 2019-03-12
• Subjects: antifungal susceptibility testing -- blood -- central venous catheter -- specific multiplex qPCR -- Wickerhamomyces myanmarensis; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0253
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: Presenting the first clinical case of Wickerhamomyces myanmarensis .Patients & methods: Yeast cells were isolated from blood and central venous catheter of a 5.5-year-old male subject. API 20C AUX, MALDI-TOF MS, ITS and LSU rDNA sequencing, and our qPCR assay were used for identification and the MIC values were determined by CLSI M27-A3.Results: ITS and LSU rDNA sequencing identified both isolates as W. myanmarensis, while API 20C AUX and MALDI-TOF MS did not identify them correctly. Our qPCR specifically distinguished W. myanmarensis from W. anomalus . Isolate obtained from blood showed a higher MIC value for fluconazole, voriconazole and posaconazole.Conclusion: Utilization of reliable identification tools might reveal the genuine spectrum of opportunistic yeast species.